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| Among the methods of assisted reproductive technology, the Intrauterine Insemination (IUI) is one of the most favorable and effective treatment. Artificial insemination with a washed sperm concentrate avoiding of the potential reaction to bacteria, proteins and prostaglandins in seminal fluid, will be performed by depositing sperm directly into the uterus on the day of ovulation. |
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| This method is appropriate for females without occlusion of both oviducts. Artificial insemination has been used to overcome ejaculatory problem, abnormal sperm quality and female cervical factor. |
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| The intrauterine insemination is a comparatively less costly method, and in general, most women will be conceiving within 3-6 insemination cycles. |
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After liquefaction, the semen is mixed with culture medium (1:1) and centrifuged at 300-400 g for 10 minutes. The supernatant is discarded and pellet is suspended in 2 ml of culture medium. This is centrifuged again at 300-400 g for 5-10 minutes and supernatant against discarded. The final pellet is suspended in 0.4-0.5 ml of medium and immediately used for insemination. |
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